Dr. Girlfriend / Henchman 21 (voice)
Hank Venture / The Monarch (voice)
Brock Samson (voice)
Dr. Venture (voice)
Dean Venture (voice)
Dr. Orpheus (voice) / Vincenzo (voice)
Dr. Jonas Venture (voice) / Jonas Venture Sr. (voice)
Triana Orpheus (voice) / Jeanie / Leslie Cohen / Lesliebots (voice)
The Alchemist (voice) / Dog-Man (voice)
Molotov Cocktease (voice) / Sally Impossible (voice)
Jefferson Twilight (voice) / Mandalay (voice)
Action Johnny (voice) / Surgeon / OSI Scientist / SPHINX Agent 1 (voice)
Rocco (voice) / Stars & Garters / Tosh Tompkins / Enzo (voice)
Sirena (voice) / Stab Girl / Mrs. Fitzcarraldo (voice)
Wide Wale / Manolo (voice) / Wide Wale / Jägermeister / Janitor (voice)
Lt. Anna Baldavitch / Woman on Cell Phone (voice) / Tijuana Prostitute (voice)
Redusa (voice) / Nikki Fictel (voice)
Myra Brandish (voice) / Val (voice)
S-464 (voice) / Phineas Phage / Prisoner 1 / The Termite / Guild Sniper (voice)
Baron Ünderbheit (voice) / Baron Ünderbheit / Otto Aquarius (voice)
Professor Impossible / Phage (voice) / Phineas Phage / Headshot / Flying Squid (voice)
General Treister (voice) / Copycat (voice)
Snoopy (voice) / Corporal Snoopy (voice)
Fallen Archer (voice) / Monseñor / Bouncer / Wonderboy 5 (voice)
