Peter Parker / Spider-Man (voice)
Mary Jane Watson (voice)
Felicia Hardy (voice)
Harry Osborn (voice)
J. Jonah Jameson (voice)
Joe "Robbie" Robertson (voice)
Kingpin (voice)
Aunt May Parker (voice)
Dr. Curt Connors (voice) / Dr. Curt Connors / Lizard (voice)
Michael Morbius (voice) / Morbius (voice)
Anna Watson (voice)
Alistair Smythe (voice) / Ultimate Slayer Smythe (voice)
Norman Osborn (voice) / Norman Osborn / Green Goblin (voice)
Debra Whitman (voice)
Flash Thompson (voice)
Detective Terri Lee (voice)
Landon (voice)
Doctor Octopus (voice) / Otto Octavius / Doctor Octopus (voice)
Madame Web (voice)
Newscaster (voice) / News Reporter (voice)
Shocker (voice) / Man-Spider (voice)
Red Skull (voice) / Beyonder (voice)
Eddie Brock / Venom (voice) / Eddie Brock (voice)
Hobgoblin (voice) / Jason Phillips / Hobgoblin (voice)
