Peter Parker / Spider Man (voice)
Gwen Stacy (voice)
Flash Thompson (voice) / Flash Thompson / Car Thief #1 (voice)
Sally Avril (voice) / Betty Brant (voice)
Liz Allan (voice) / Liz Allan / Trina (voice)
Mary Jane Watson (voice) / Mary Jane Watson / Rosie Thompson (voice)
Harry Osborn (voice) / Harry Osborn / Frederick Foswell (voice)
May Parker (voice)
George Stacy (voice) / Rhino / George Stacy (voice)
Norman Osborn (voice)
Rand Robertson (voice) / Rand Robertson / Robbie Robertson (voice)
Eddie Brock (voice) / Venom (voice)
J. Jonah Jameson (voice) / J. Jonah Jameson / John Jameson (voice)
Tombstone (voice) / Principal Davis (voice)
Otto Octavius (voice) / Doctor Octopus (voice)
Hammerhead (voice) / Flint Marko / Hammerhead (voice)
Curt Connors (voice) / Curt Connors / Homunculi (voice)
Glory Grant (voice) / Glory Grant / Cobweb / Homunculus #2 (voice)
Electro (voice) / Max Dillon / Electro (voice)
Vulture (voice) / Adrian Toomes / Vulture (voice)
Doc Bromwell (voice)
Black Cat (voice) / Felicia Hardy / Black Cat (voice)
Silvermane (voice) / Silvio Manfredi / Silvermane (voice)
Sergei Kravinoff / Kraven the Hunter (voice) / Kraven (voice)
