Presented in Supercolorisation

Dr. Horatio Beaker / Mitch the Monkey / Herman Gredenski (voice) / Professor Matthew Matic / Lieutenant Ninety / Freighter A14 (voice)

Doctor Venus (voice) / Dcotor Venus (voice)

Colonel Steve Zodiac (voice) / Colonel Steve Zodiac / Mrs Doughty (voice)

Robert the Robot (voice) (uncredited) / Robert the Robot / Granatoid 1 (voice) (uncredited)

Commander Wilbur Zero / Kazak (voice) / Commander Wilbur Zero / Jock Campbell (voice)

Mike Mercury / Jason Munroe III (voice) / Mike Mercury / Film Narrator (voice)

Professor Rudolph Popkiss (voice) (credit only) / Professor Rudolph Popkiss / Black Morgan (voice)

Sheriff Tex Tucker / Telegraph Operator Dan Morse (voice)

Dusty the Dog / Rocky the Horse / Foreman (voice)

Martha 'Ma' Jones (voice)

Sheriff Tex Tucker (singing voice) (uncredited)