Indira 'Indy' Daimonji (voice) / Indira 'Indy' Daimonji (voice)
Peter Parker / Spider-Man (voice)
Mary Jane Watson (voice)
Harry Osborn (voice)
Alison Tomita (voice)
Officer Barr (voice)
J. Jonah Jameson (voice)
Doug's friend (voice) / Muang / Drip Den guy (voice)
Max Dillon / Electro (voice) / Max Dillon (voice)
Sally Johnson (voice)
Silver Sable (voice)
Kraven the Hunter (voice)
Sergei (voice)
Raymond (voice) / Policeman (voice)
Dr. Zellner (voice) / Professor (voice)
Shadowy Woman / Flower Vendor (voice) / Head Protestor (voice)
Roland Gaines (voice)
Roxanne Gaines (voice)
Female Reporter (voice) / Female News Announcer (voice)
Wilson Fisk / The Kingpin (voice)
Frank Elson (voice)
Dr. Curt Connors / The Lizard (voice)
Shikata (voice)
Richard Damien (voice)
